BIONB 4300

BIONB 4300

Course information provided by the Courses of Study 2022-2023.

Experiments include PCR, western blots, RNAi, antibody staining, optogenetics, calcium imaging of neurons and behavioral assays. Students will also construct their own epifluoresence microscope. Experiments emphasize how molecular techniques can be applied to studying neurobiological problems and will culminate in designing your own experiments. It is critical to be present at the lab from 9am- 11am and then again from 1pm-2pm as the experiments have incubation steps at the beginning and the experiments must be evaluated at the close of class. However, if students would like to take a conflicting course in the 11am-1pm range on Tuesdays, the instructor will support a time conflict approval form. A discussion section on Thursdays will also be scheduled after the course begins. Instructor will work with students to find an agreeable time on Thursdays. If you have additional questions please contact me at

When Offered Spring.

Prerequisites/Corequisites Prerequisite: BIOMG 3300 or BIOMG 3310. Recommended prerequisite: BIOMG 2801.

Distribution Category (BIO-AS, PBS-AS)

  • Demonstrate competence in basic molecular biology and protein biochemistry skills in the laboratory and the underlying principles of these techniques.
  • Demonstrate mastery of basic bioinformatics tools such as BLASTN, TBLASTN, and BLASTP and using model organism databases such as FlyBase.
  • Develop a clear understanding of how different laboratory techniques provide complementary information about gene expression.
  • Demonstrate deep understanding of scientific procedures and results through written laboratory reports.

View Enrollment Information

  •   Regular Academic Session. 

  • 4 Credits Graded

  •  1822 BIONB 4300   LAB 401

    • T Wing Hall 302
    • Jan 23 - May 9, 2023
    • Deitcher, D

  • Instruction Mode: In Person
    Prerequisite: BIOMG 3300 or BIOMG 3310. Recommended prerequisite: BIOMG 2801. The lab is open until 5:00 pm for additional work. Labs frequently last to 1 or 2 PM but it is possible to attend one class on Tuesday if it meets after 11 AM as the experiments have incubation steps or other breaks. But plan on returning after class to finish the experiments. A section on Thursday will be scheduled after the course begins. If you have additional questions please contact me at Lab will be held in WING 302.