PLBIO 4831

Concepts and Techniques in Plant Molecular Biology

Course Description

Course information provided by the Courses of Study 2015-2016.

Introductory module that provides a broad overview of molecular biology concepts relevant to the plant sciences. Serves as a prerequisite to other modules in the BIOPL 483X (fall) and BIOPL 482X (spring) classes. Course covers genetic concepts relevant to molecular markers, map-based cloning, insertional mutagenesis, forward and reverse mutant screens, analysis of transcriptomes, organelle and nuclear genome structure, and transformation. Techniques include nucleic acid manipulation, blot and in situ hybridization, chromatin immunoprecipitation, high throughput sequencing, PCR, microscopy, laser microdissection, microarrays, metabolomic profiling, proteomics, protein-protein interactions, electrophoresis, and immunological methods.

When Offered Fall.

Prerequisites/Corequisites Prerequisite: BIOMG 2800 and BIOMG 3300 or BIOMG 3320, or equivalents. Recommended prerequisite: BIOMG 3310.

• The overall objective of this course is to bring students from diverse backgrounds and interests to a common level of understanding in preparation for subsequent courses and research in areas related to plant molecular biology. By the end of the semester, students should be able to explain, give relevant examples of, and make predictions based on their understanding of how: fundamental concepts from biochemistry, molecular and cell biology, genetics and plant biology have been and can be integrated in their application to modern plant molecular biology modern experimental techniques in biology and related fields have been and can be applied to relevant questions in plant molecular biology limitations of these experimental techniques constrain the interpretation of experimental data. In addition, students should become more adept at objectively reading and evaluating current literature, particularly in the context of concepts and techniques discussed in the course.

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