BIOMG 4400

Laboratory in Biochemistry and Molecular Biology

Course Description

Course information provided by the Courses of Study 2014-2015.

Experiments related to molecular biology (includes PCR, DNA cloning, hybridization analysis, restriction mapping, and DNA sequence analysis), protein purification and analysis (salt fractionation, ion exchange chromatography, affinity chromatography, SDS-PAGE, and immunoblotting), and determination of enzyme kinetic parameters.

When Offered Fall, spring.

Permission Note Limited enrollment.
Prerequisites/Corequisites Prerequisite: BIOMG 3300, or BIOMG 3330, or BIOMG 3350, or BIOMG 3310/BIOMG 3320 (at least one of BIOMG 3310/BIOMG 3320 completed but one may be taken concurrently). Priority given to undergraduate biology majors in Biochemistry or Molecular and Cell Biology programs of study and to graduate students with a minor in field of biochemistry.

Distribution Category (PBS)

• After completing BIOMG 4400, students should be able to: Plan, carry out, and interpret the results of laboratory experiments.
• Use modern laboratory techniques to purify and study proteins and to purify and work with nucleic acids.
• Explain the theoretical basis of the laboratory techniques.
• Use Internet databases to assist in data analysis.
• Create and use a laboratory notebook.
• Present results of laboratory work, both orally and in writing.

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